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Excellent microscope introduction, resources and advice
Macro links
Focus stacking
Some microscope optics basics
Photomicrography with an infinity-corrected microscope - Macro Cosmos
Practical guide to binocular colimation - Ron Green
Killing specular reflections with polarizing filters
Camera + Microscope Combinations

Labophot 1
Nikon Metaphot
Optiphot 1
Optiphot 66
Optiphot 66 DIC

AO Series 10 - headless
AO/Reichert EPIStar
AO Series 120

Anker USB charger and power bank
AO PhotoStar hack
copy stand
Dolan-Jenner Fiber-Lite Model 180
First surface mirror
Focusing helicoids
LED illumination
M42 iris
Monolux Astro/Micro-Photo Adapter
M42 to 30mm to 23.2mm eyepiece adapter
MTF targets
Nikon 210mm tube length objectives
Nikon trinocular heads
Novoflex EOS-RETRO Reverse Lens Adapter
Optem Optizoom 0.8x-2x
Pinspot RGBW 10W LED
Zeiss flip top 0.9 condenser

Microscope Stylus Images


Photomicrography and macro photography are both popular subjects in the InterWeb,
including among better writers than I.  Consequently, content here will focus (heh) on specific experiences,
rather than more general theory and technique, which for macro photography see e.g. Allan Walls.

Years ago, I saw and admired Micrographia and more recently Ray Parkhurst's work
including contributions to Stylus images on the not cheap (but not massively expensive).
Capturing images of phonograph needles combines 3 of my long-time interests:
audio, photography, and microscopy, although mostly microscopes with cameras.

While not aspiring to create aesthetically pleasing images,
facilitating capture of clear and useful phono stylus image wear images seems achievable.
Ray demonstrated that Nikon's long working distance CF objectives are well-suited to this,
while I already owned American Optical (AO) microscopes lacking long working distance objectives, oh well.


Microscopes traditionally shared some mechanical conventions: Consequently, optically-matched objectives and oculars could be swapped among frames.
Practically, after frames evolved from straight tubes to separate heads with prisms,
additional lenses might be introduced, specifically to allow for e.g. vertical illuminators
and/or polarizing filters to be inserted into optical paths.
These optics are typically optimized for some manufacturer's objectives optical formula.
Most modern microscopes use other than RMS threads; here is a list.
Here is a list of threads used in astronomy.

Camera + Microscope Combinations

Brooke Clarke also addressed these combinations.

Attaching microscope objectives directly to cameras is considered macro photography,
rather than photomicroscopy, and will mostly be addressed elsewhere.
However, attaching a camera body to a microscope body
with nothing but air between objective and lens IMO qualifies as photomicroscopy. This works with so-called finite objectives.
Infinity objectives want a so-called tube lens to make rays finite,
either for oculars or directly focusing on a sensor, in which case
that tube lens may be a telescopic camera lens focused at infinity.
AO pioneered infinity microscope production in the U.S.;   read here for more history
Practically, for optical magnifications much more than 1x,
technique and patience exceeding mine are wanted for hand-held macrophotography.
For inanimate as well as opaque subjects, hand-holding has no advantage.

Opaque object microscopy requires reflective, rather than transmissive, illumination.
This is called episcopic, rather than diascopic, with illumination accomplished either by
external illumination sources or using so-called vertical illumination built into microscopes,
where light may be directed down thru objectives, e.g. using prisms or other partial mirrors,
or coaxially around objectives, called darkfield illumination.

Most conventional (diascopic biological) microscopes can be used with external illumination
for some opaque microscopy, but their objectives are optimized for viewing thru glass slide covers,
which becomes increasingly important for higher magnification objectives, e.g. more than 10-20x.

AO PhotoStar restomod

In which parts of a dedicated camera solution become a generic M42 camera adapter.

baby steps with EPIStar

Initial stylus image capture employs a 10x epi microscope objective with darkfield illumination and Olympus NFK 3.3x projection ocular into a Canon EOS sensor.
To hold the stylus at ~45 degrees, the cartridge was mounted in a headshell with no finger lift and clamped at the bayonet connector using a clothespin:

Although it facilitates sorting spacing between camera sensor and objectives, a bellows is not necessary;
extension tubes suffice, with more length yielding greater magnification.
The resulting image has decent resolution, but poor contrast.

This microscope has short working distance objectives;
the 20x objective would not clear this stylus' plastic grip at this angle.
90 degree shots will clear but require image stacking;
whether objective darkfield illumination will then highlight stylus wear spots remains to be seen.

Live View, focus bracketing, Abbe

Using a camera with zoomed Live View eases focusing.
Beyond convenience, best viewfinder focus with DSLR mirror down may differ from best focus on the sensor.
Silent shutter (Canon's mode 2) and remote shutter release minimize vibration during exposures.
Replacing halogen illumination with blue LEDs should improve image resolution,
since the Abbe diffraction limit depends on illumination wavelength and Numerical Aperture (NA).
Practically, blue LEDs not only have shorter wavelengths than do red and green
but excluding those longer wavelengths also usefully reduces chromatic artifacts from older and simpler lenses.
Canon DSLRs with Live View and silent shutter (e.g. 50D) can be had used for less than $100.
Even better, free Magic Lantern firmware adds focus stacking for many such cameras.

eyetube camera adapter clamps

Traditional eyepiece tubes have about 25mm o.d., for which T-mount adapters were made. Common brands were Kalt, Aetna and Telestar, but many identical or similar clamps were branded for cameras.

For larger o.d. eyepiece tubes, up to 1.25" (less than 32mm), a telescope clamp can work:

... perhaps with shims:

Eyetube adapters typically have T2 or M42 threads, requiring a camera-specific adapter.
A projection ocular and extension tubes should cost less than $100;
monocular microscope heads typically cost much less than trinoculars,
while having less light loss than binocular heads,
and used eyetube camera adapter clamps go for around $15.
obtaining a more or less complete used microscope with epi illumination and bright/darkfield objectives can be tricky.
Starting from scratch, consider Nikon 210mm B/D objectives in an Optiphot...

EPIStar contrast

To investigate low contrast, Ray Parkhurst suggested removing the camera and looking down into the tube.

Internal surfaces were not reflecting appreciable light,
but a small bright spot and dim blob are seen in the optic at the bottom of the eyetube.

Lacking ground glass, wax paper was laid over the camera adapter:
The dim blob is revealed as the projected stylus.
The bright spot appears less focused than when viewed directly and,
depending on viewing angle, more or less distinct from the projected stylus image.

Removing the microscope head and looking down into the arm
reveals a bright spot centered in an epi illuminator lens
in a port off to one side
as well as a less bright spot on the lower lip of that port.
That very bright area below the epi lens is the brightfield mirror.

An A.O. #3002 image erector spaces the head further from the epi illuminator
and slightly improved contrast, despite adding magnifying optics in the path:

EPIStar 10X with EF-S 55-250mm STM

Infinity objectives can be used with a camera lens focussed @ infinity,
in this case, a Reichert EPIStar 10x with Canon EF-S 55-250mm STM zoomed to 250mm.
To prevent this zoom from collapsing to 55mm, the camera body needs support.
Fortunately, focus is internal. Some observations:

PE 2.5 suspended with bellows and Canon 6D
over pasteboard tube to AO trinocular head

AO Series 10 - headless

Disappointed with EPIStar images, Series 10 configuration experiments resumed.

RGB ring light

Microscopes better resolve by blue illumination, since shorter wavelength and less opportunity for chromatic aberrations.
A 40mm RGB LED halo ring fits around microscope objectives and can be supported by a 20mm i.d. o-ring.

That o-ring below LED halo blocks some illumination...

Since Bayer-filtered camera sensors have relatively sparse blue pixels,
aliasing is liable for images with less than 4x oversampling.

The dark wedge along the center of the above blue M55E stylus image is partly surface not in focus,
but also stylus angle not 45 degrees (twisted cantilever).

The next revision glued a curled strip of business card as a half-cylinder to the halo backside...
...allowing an o-ring to secure LEDs on the objective from above:

Rotating the headshell in the clothespin to bring more of the stylus upper surface more nearly in focus
and using green light instead of blue to involve more camera pixels yields the following:

Contrast could be improved by putting something flat black between the cantilever and its light plastic grip.


Microscope fine focus is indexed in microns, 200 per revolution.
Images of 0.01mm test slide (with cover slip) using MEIJI S.PLANM 20X with Series 10 tube lens and Olympus PE 2.5X; no matching corrections.
red 144green 153
white 148blue 153
Red focus is out about 9 microns from blue and green..

finite focal length objective

A set of infinity objectives acquired years ago
also included a LEITZ WETZLAR 160/- EF 10/0.25
with 6.8 mm working distance (Laborlux K-D brochure page 14)
A recently acquired RMS to T2 adapter enables macrophotography experiments.
Having been attached using hot glue,
the RGB LED ring light mounting sleeve failed
during an extended session;
illumination temporarily reverted to white LED gooseneck
while epoxy cures.
Practically, focusing a 10X objective by bellows
is less fun than by headless microscope.


Increased spacing between objective and focal plane
in theory impacts numerical aperture,
but trials with bellows did not indicate any drastic effects,
with results compromised by difficulty focusing.
Eliminating all optics but objective should increase contrast,
but illumination differences and reduction in magnification confound comparison.

Here is a 1:1 crop of that stylus tip from an APS-C Canon sensor:

With camera body attached to a microscope, zoomed live view eases focusing,
but using objectives with bellows mostly demonstrated
limitations of conventional photography focus rails.
Resolution in this setup may also be constrained by sensor pixel density..

50.5mm dovetail to M42 adapter arrived from RAF Camera.
This secures camera body and extender tubes to A.O. arm without head
(and tube lens).
Finite objectives can then be used in A.O. arm
with much better positioning and focus control than by bellows.
10X is not nearly enough to fill an APS-C sensor with stylus:

Making this image was much easier than with bellows,
except for positioning the LED to approximately match illumination:

Leitz color calibration on Series 10 with 7D

white blue green red
74 65-8 72-5 67-70

Field of view: full frame 20X and 3.3X relay vs APS-C 10X

These are both 480*320 downsamples of full sensor images in the same microscope arm with similar ring illumination:
Leitz 10X 160mm and APS-C sensorMEIJI 20X infinity, A.O. tube lens, 3.3X relay and 35mm sensor
Zooming the Leitz image
(right-click, "Open image in new tab", then [Ctrl]&[+] keys)
reveals that only about the center third is nearly focussed;
this objective wants to be used with a 3.3X relay lens..
.. or perhaps 2x telextender.

Leitz 10X with NFK 3.3X

Repurposing available bellows, EF and T2 extension tubes with adapters
yields nearly 140mm parfocal distance for Leitz objective to NFK 3.3X relay lens
and 150mm projection distance to APS-C sensor.

NFK 3.3X is perched at the T2-EF adapter:

This stack is decidedly less than rigid
and really needs electronic shutter and remote release.

Field of view is usefully improved: (downsampled but uncropped)
blue 109"white" 107
red 97green 101
NFK 3.3X corrections designed for Olympus objectives cause chromatic focus differences from Leitz 10X alone.

With 40mm RGB LED halo on Leitz objective, M55E stylus is better illuminated.
Additional 3.3X magnification allows use of blue LED for better optical resolution
followed by about 4x downsampling after converting blue to grayscale and adjusting dynamic range:

The stylus in the above image was rotated less than 45 degrees from horizontal.
This image has the stylus rotated somewhat more than 45 degrees,
illuminated by white (R+G+B)LED halo, no contrast adjustment:

Ocular Swap

When using infinity objectives, swapping between regular eyepiece and camera on monocular head is fairly quick and easy:
1/4 turn releases camera tube remove relay lens insert eyepiece, find stylus
Reverse steps to resume image capture.

A.O. 10X with NFK 3.3X

Finding the stylus with a microscope objective is easier using conventional oculars
than with camera Live View and projection ocular.
Since the head has an infinity tube lens, this requires infinity objectives.
Since Series 10 AO Spencers often include a 10X objective, how well does one work on a stylus?
Because they are smaller in diameter than MEIJI and Leitz objectives
for which cardstock half-cylinders were epoxied to LED halos, a shim was cut from clear vinyl tubing.

This particular objective appears to have about 4mm working distance.
With halo illumination, stylus tip is better illuminated with cartridge vertical centerline
rotated more nearly 60 than 45 degrees from horizontal, reducing stylus surface in focus.

With NFK 3.3X relay lens and about 200mm projection distance,
full field of view with APS-C sensor is usefully in focus;
both of these images (downsampled, but not cropped) were captured after central focusing (zoomed Live View):

After visually confirming that minimal detail is lost by downsampling original (5472x3648) images by 3x,
then, given optics are NOT matched for color correction,
compared whether blue (left) or green (right) illumination yields more detail:
Approximate IrfanView color corrections settings for recovering dynamic range after grayscale conversion
and before 3x downsampling: brightness ~25, contrast ~99, gamma ~1.65

Leitz objective has working distance and nose profile for 45 degree illumination.

Calibration shot with Leitz EF and 3.3X photo relay to APS-C sensor:

Much of the camera sensor is wasted on out-of-focus objective.

Reducing blue images from 5472x3648 to 800x533
imperceptibly impacts detail:

...while green images noticeably lose detail below 1280x853:

relatively sparse blue pixels in Canon sensor's Bayer matrix
evidently matter more than light wavelengths at this lower magnification.

Introducing a (white) "helper" LED:

Rotating M55E stylus from 45 to 60 degrees improves illumination

...because of twisted stylus:

Pickering D400 stylus shows more typical 45 degree illumination:

USB-powered phono headshell servo

RC Servo Tester wants 4.8-6.2VDC
provided by splicing it to a USB cable.

A short section of elastomer tubing couples that servo to a headshell's connector
and allows tweaking to align the phono stylus with servo axis.
The white card provides a reference for grayscale calibration of blue illumination.

Servo tester rotates the stylus for optimal illumination angle.

AO 1053 35mm camera back flange with 1057 adapter

AO camera flange registers inside M42 camera adapter ring.